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Real-time PCR is a technique for amplifying and detecting RNA/DNA of fish pathogens (VHSV, Marine birnavirus, Turbot iridoviurs, Nervous necrosis virus, Streptococcus sp., Edwardsiella sp., Pseudomonas sp., Vibrio sp., and so on)

Bacteriological examination may detect any bacteria growing under specified conditions (selective agars, temperature, and so on) and identify the bacteria with biochemical tests

Bacterial agglutination test can measure the specific antibodies produced by the host against bacterial agglutinins